9.1. Production of QPM: Standardization of protocol for vegetative propagation of some high valued and threatened medicinal plants of Odisha.
- Production of QPM of Operculina turpethum through vegetative propagation
Production of QPM of Operculina turpethum through vegetative propagation
In order to facilitate mass QPM production of Operculina turpethum, vegetative propagation methods using different microcuttings viz. single node with juvenile shoot bud, single node with axillary shoot were standardized apart from conventional nodal stem cuttings (10 cm length with two nodes and two leaves). These nodal cuttings were treated with selected hormonal treatment and planted in poly pots containing sand: soil: compost in 1: 1: 1 as rooting medium. Besides, some nodal cuttings were planted in root trainers having soilrite as synthetic rooting medium and kept under mist house for rooting.
In all the cases, root initiation (3-5 roots) was observed after 7-10 days of treatment. It was found that production & development of roots was higher when cuttings treated with hormones in comparison with non-treated ones.
Rooting efficiency of Operculina turpethum nodal cuttings was found promising in T1 treatment (IBA 2000 ppm + NAA 3000 ppm) as compared to the control (untreated cuttings). Highest root length found was 10.61 cm. with 11 nos. of roots on an average. Lowest root length found was 3.5 cm. with 4 nos. of roots on an average. Highest percentage of survivability was 81.48% in T1 treatment.
Field Trial: Establishment of vegetatively propagated QPM of O. turpethum
The successfully raised, primary hardened plantlets of Operculina turpethum were transferred to open nursery for secondary hardening where the saplings were prepared ready for outplanting in sampling plots. The outplanting was done in experimental plots of 25 ft × 30 ft size at a spacing of 60 cm × 60 cm. using normal compost and cowdung soil mixture .
Roots and Fruits/seeds were harvested and recorded during first and second year of maturity for production analysis (details to be reported later).
Field-establishment and production trial of vegetatively propagated QPM of Operculina turpethum:A-mature plant in trial plots;B-Fruiting and seed setting;C-Harvested seeds